hemagglutination inhibition assay

HAI is one of the most commonly used methods to quantify immunity from influenza and other . Latex beads coated with a specific antibody are agglutinated in the presence of homologous antigens (bacteria). The basis of the HAI assay is that antibodies to that particular virus (for example; measles virus- see image) will prevent attachment of the virus to RBC. This can have a significant effect on the hemagglutination-inhibition test since antibody titers measured by this procedure are related to the amount of antigen used. The hemagglutination inhibition (HAI) assay represents the gold standard method for measuring the functional antibody response elicited following standard-of-care vaccination, along with evaluating the efficacy of under-development influenza vaccines in both animal models and clinical trial settings. Since 1964, seroepidemiologic studies with the hemagglutination inhibition test (HI) have been conducted routinely for clinically suspected cases. Supplemental Assay Method for Conducting the Hemagglutination Inhibition Assay for Equine Influenza Antibody UNCONTROLLED COPY 2.2.10 Test Virus Each manufacturer provides each strain of type A equine influenza virus present in the Test Serial that has been correlated to protection in a host animal immunogenicity trial. All serum samples, including the sheep serum samples and negative control, were pre-treated with receptor-destroying enzyme (RDE) (ratio 1:5) from Vibrio Cholerae (Sigma-Aldrich, Milan, Italy) for 18 hours at 37°C in a water bath and then heat-inactivated for 1 hour at 56°C in a water bath with 8% sodium . Reaction the mixture was centrifuged and the serum For influenza virus, HI assay is used to identify the hemagglutinin (HA) subtype of an unknown isolate or the HA subtype specificity of antibodies to influenza virus. Hemagglutination Inhibition Test (HAI) for Avian Influenza; Quantitative Micro Hemagglutination Test (HA) Latex agglutination test for Ag detection. Thus, 6 different HI assays The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. JAALAS accepts research reports (data-based) or scholarly reports (literature-based), with the caveat that all articles . However, the microneutralization (MN) assay has been reported to have higher sensitivity when testing sera from humans and other species. The hemagglutination inhibition (HI) assay is commonly used to assess the humoral immune response against influenza A viruses (IAV). The HA inhibition assay - YouTube The hemagglutination titer is the highest dilution of serum that completely inhibits hemagglutination by a standardized viral preparation. Hemagglutination Inhibition - an overview | ScienceDirect Topics BACKGROUND: Serum antibody to influenza can be used to identify past exposure and measure current immune status. Haemagglutination inhibition test (HI) - YouTube The HI assay was performed according to the standard procedure, 26 using 0.5% chicken erythrocytes and 4 hemagglutinating units of SwH1N1.

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